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KMID : 0370219970410030381
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Yakhak Hoeji
1997 Volume.41 No. 3 p.381 ~ p.388
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in vivo and in vitro Metabolism of Recombinant Human Epidermal Growth Factor (DWP401) in Rats
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고여욱/Koh YW
남권호/정주영/박승국/유영효/김재환/한건/박명환/심창구/Nam KH/Jung JY/Park SK/Yu YH/Kim JH/Han K/Park MH/Shim CK
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Abstract
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Metabolism of DWP401, recombinant juman epidermal growth factor, was examined in vivo and in vitro in rats. When 125I-labeled DWP401 was administered at a dose of 50 mcg/kg by i.v. injection. 125I-labeled DWP401 was rapidly degraded within 30 minytes above 93%. Thin layer chromatography analysis of urine collected for 24 hr after i.v. administration of 125I-labeled DWP401 showed ohly one spot on a X-ray film which was considered as diiodo-tyrosine. This result suggests tha 125I-labeled DWP401 was completely digested into free amino acids without any specific intermediate polypeptides. About 42.1% of the administered iodine was recovered in 24 hr. For in vitro degradation study, 125I-labeled DWP401 was added to plama and tissue homogenates of rats and incubated at 37oC. Almost 98% of the added radioactivity recovered from the protein fraction of the liver, kidey, small intestine, stomach and spleen decreased rapidly. For examplem the recovery rates of 125I-labeled DWP401 were 58.6, 63.2, 39.9, 52.9 and 66.8% after 4hrs of incubation in respective organ homogenates.
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